瓊(qiong)脂糖(tang)預染(ran)預制(zhi)膠(jiao)說(shuo)明(ming)書(shu)

*Shipping and Storage

瓊(qiong)脂糖(tang)預染(ran)預制(zhi)膠(jiao)電泳(yong)試(shi)劑盒的小(xiao)黑(hei)盒 2~8℃保(bao)存(cun)和(he)運(yun)輸(shu)，有(you)效(xiao)期 12 個月(yue)。

Ship and store the kit at 2~8℃. It will remain stable for one year.

專適 6×DNA Loading Buffer 2~8℃運(yun)輸(shu)，長期需要(yao)-20℃保(bao)存(cun)，有(you)效(xiao)期 12 個月(yue)。

Ship Optimized 6×DNA loading buffer at 2~8℃，for short-time storage and at -20℃ for long-time storage. It will remain stable for one year.

TAE 速溶(rong)顆粒(li) 2~8℃或常(chang)溫運輸(shu)，常(chang)溫保(bao)存(cun)，有(you)效(xiao)期 24 個月(yue)。

Ship TAE Instant Granule at 2~8℃ or room temperature and store at room temperature. It will remain stable for two years.

專適 Marker 2~8℃運(yun)輸(shu)，長期需要(yao)-20℃保(bao)存(cun)，有(you)效(xiao)期 12 個月(yue)。

Ship Optimized Marker at 2~8℃ and store it at -20°C for long-time storage. It will remain stable for one year.

*自備試劑(ji)

*Reagents Required But Not Provided

核酸樣品、去(qu)離(li)子(zi)水(shui)

Nucleic acid sample and Deionized water

*使用(yong)方(fang)法

*Procedure

1.量取約(yue) 600ml 的蒸(zheng)餾水(shui)加(jia)入燒杯(bei)，並放(fang)置壹(yi)個磁(ci)性(xing)攪(jiao)拌(ban)子(zi)於(yu)燒杯中。將燒杯(bei)置於(yu)磁力(li)攪(jiao)拌器上，慢(man)慢(man)加入 1 袋 TAE 速(su)溶(rong)顆粒(li)的全(quan)部內容(rong)物(wu)，攪拌(ban)溶(rong)液直(zhi)至

溶(rong)解。把(ba)燒(shao)杯(bei)中(zhong)的溶(rong)液倒入 1000ml 的容(rong)量(liang)瓶(ping)中，再(zai)加(jia)入蒸(zheng)餾水(shui)，定(ding)容(rong)至 1000ml，即(ji)為(wei) 1×TAE 溶(rong)液。

1. Add one pouch of TAE Instant Granule into the cleaned beaker, dissolved completely with 600 ml distilled water under a magnetic stirrer. Pour the solution into 1L flask. Add distilled water to the

solution until the total volume is 1L. The final solution is 1×TAE buffer.

2.取(qu)出壹(yi)塊獨立(li)包(bao)裝的瓊(qiong)脂糖(tang)預染(ran)預制(zhi)膠(jiao)，撕(si)掉表面(mian)的塑(su)料膜(mo)，反(fan)轉(zhuan)包裝，用(yong)兩(liang)手的食(shi)指(zhi)和(he)中(zhong)指(zhi)托(tuo)住(zhu)塑(su)料殼(ke)邊(bian)緣，開口向(xiang)下沒入電泳(yong)液(ye)中，然後用(yong)兩(liang)個大(da)拇(mu)指(zhi)輕

輕按壓(ya)塑料殼(ke)背(bei)面(mian)中心(xin)部(bu)分，瓊脂糖(tang)預染(ran)預制(zhi)膠(jiao)就會落(luo)入電泳(yong)液(ye)中，此時(shi)的預(yu)制膠(jiao)帶孔面(mian)向(xiang)上(shang)，移動膠(jiao)塊，使孔側端(duan)靠近(jin)電(dian)泳(yong)槽(cao)負(fu)極。如樣品孔內有氣(qi)泡，應(ying)設(she)法除

去。

2. Take out one kit, take off the plastic package, reverse it, support the two edges with index and middle fingers of both hands, immerse it in the buffer with the opening downward and gently press the

central part of the kit with two thumbs. Thus the gel will fall into the buffer with the side of the well upward. Move the gel to make the well end close to negative electrode of the electrophoresis cell. If

bubbles are produced in the sample wells, try to remove them.

3.按 5:1 的比(bi)例取(qu)適量(liang)核(he)酸樣品和(he)專(zhuan)適 6×DNA Loading Buffer 混(hun)勻(yun)，用(yong)移液器將專適 Marker 和(he)樣品混(hun)合(he)液依(yi)次緩慢(man)加入被(bei)浸(jin)沒的凝(ning)膠(jiao)加樣孔內。

3. Mix Optimized 6×DNA loading buffer and DNA sample at a volume ratio of 1:5. Carefully load prepared Marker and the mixed sample into the wells with pipette successively.

4.接通電源(yuan)，紅(hong)色為(wei)正極，黑(hei)色為(wei)負(fu)極，切(qie)記 DNA 樣品由負(fu)極往(wang)正極泳(yong)動(dong)(靠近(jin)加(jia)樣孔的壹(yi)端(duan)為(wei)負(fu))。

4. Connect the electrophoresis cell to the power source according to the conventions: Red-Anode and Black-Cathode. Turn on the power source. Note that the DNA sample moves from the negative to

the positive (the end near the wells that DNA samples are loaded in is negative).

5.根據(ju)指(zhi)示(shi)劑(ji)泳(yong)動(dong)的位置，判斷是否終(zhong)止(zhi)電(dian)泳(yong)。

5. Determine whether to stop electrophoresis according to the migration of the tracking dyes.

6.電(dian)泳(yong)完(wan)畢(bi)，關(guan)閉(bi)電(dian)源(yuan)，用(yong)凝(ning)膠(jiao)成像(xiang)儀(yi)觀(guan)察(cha)電(dian)泳(yong)條(tiao)帶及(ji)其(qi)位置，與(yu) Marker 比(bi)較擴增產物(wu)的大(da)小(xiao)。

6. Switch off the power source when the electrophoresis finishes. Visualize the band by using a gel documentation system and compare the size of the amplified product with that of Marker